伊人色综合一区二区三区,亚洲综合一区二区精品久久,国产激情精品视频二三区,婷婷色综合久久

類(lèi)器官Endometrial Cancer Organoid Kit

簡(jiǎn)要描述:類(lèi)器官Endometrial Cancer Organoid Kit
類(lèi)器官(Organoids)是指將成體干細(xì)胞或多能干細(xì)胞在體外三維培養(yǎng)形成的具有一定空間結(jié)構(gòu)的組織類(lèi)似物。類(lèi)器官在組織結(jié)構(gòu)、細(xì)胞類(lèi)型、自我更新能力和功能等方面與來(lái)源組織高度一致,從而在發(fā)育生物學(xué)、疾病造模、精準(zhǔn)醫(yī)學(xué)、藥物研發(fā)、基因和細(xì)胞療法、感染和免疫以及再生醫(yī)學(xué)等生物醫(yī)學(xué)的多個(gè)領(lǐng)域展現(xiàn)出*的優(yōu)勢(shì)。

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):代理商
  • 更新時(shí)間:2025-06-26
  • 訪  問(wèn)  量:801

詳細(xì)介紹

品牌其他品牌供貨周期現(xiàn)貨

類(lèi)器官Endometrial Cancer Organoid Kit


類(lèi)器官(Organoids)是指將成體干細(xì)胞或多能干細(xì)胞在體外三維培養(yǎng)形成的具有一定空間結(jié)構(gòu)的組織類(lèi)似物。類(lèi)器官在組織結(jié)構(gòu)、細(xì)胞類(lèi)型、自我更新能力和功能等方面與來(lái)源組織高度一致,從而在發(fā)育生物學(xué)、疾病造模、精準(zhǔn)醫(yī)學(xué)、藥物研發(fā)、基因和細(xì)胞療法、感染和免疫以及再生醫(yī)學(xué)等生物醫(yī)學(xué)的多個(gè)領(lǐng)域展現(xiàn)出*的優(yōu)勢(shì)。

產(chǎn)品介紹
Product Description:
bioGenousTM Endometrial Cancer Organoid Kit is a chemically defined cell culture medium for the establishment and maintenance of human endometrial cancer organoids. Patient-derived cancer organoids recapitulate the genomic and pathological features of original tumors and therefore hold great promise for medical research and precision medicine.



技術(shù)參數(shù)Product Information:

ComponentComponent Cat#VolumeStorage & Stability
bioGenousTM Endometrial Cancer Organoid Basal MediumK2166-EC-A100/A500100mL/500mL4,12 months
bioGenousTM Endometrial Cancer Organoid Supplement B (50x)K2166-EC-B100/B5002mL/10mL-20, avoid repeated freeze-thaw cycles, 12 months
bioGenousTM Endometrial Cancer Organoid Supplement C (250x)K2166-EC-C100/C5000.4mL/2mL-20, avoid repeated freeze-thaw cycles, 12 months

Preparation of Endometrial Cancer Organoid Complete Medium
Use a sterile technique to prepare the endometrial cancer organoid complete medium. The following example is for preparing 10mL complete medium. If preparing other volumes, adjust accordingly.
1.      Thaw Endometrial Cancer Organoid Supplement B(50x) and Endometrial Cancer Organoid Supplement C(250x) on ice. Mix thoroughly.
NOTE: Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.
2.      Add 200μL Endometrial Cancer Organoid Supplement B(50x) and 40μL Endometrial Cancer Organoid Supplement C(250x) to 9.76mL Endometrial Cancer Organoid Basal Medium. Mix thoroughly.
NOTE: If not used immediately, store the complete medium at 2-8°C for not more than 2 weeks. The Endometrial Cancer Organoid Supplement B contains fungicide and antibiotics (50x).
Protocol for Establishment of Patient-Derived Endometrial Cancer Organoids
CAUTION Studies involving primary human tissue material must follow all relevant institutional and government regulations. Informed consent must be obtained from all subjects before the collection of the primary human tissue material.
Establishment of Organoids from Primary Tissue
1.      Collect primary human endometrial cancer tissue pieces in ice-cold Primary Tissue Storage Solution (K601005) with conical tubes. Keep tissue samples at 4°C until the start of the isolation.
2.      Assess whether the obtained tissue pieces consist purely of epithelium. If fat or muscle tissues are present, remove these non-epithelial components as much as possible using surgical scissors or scalpels and forceps under a dissection microscope. If no fat or muscle tissues are present, continue to the next step immediately.
3.      Rinse the tissue with Cancer Organoid Basal Medium (B213152) or DPBS twice.
4.      Mince the tissue into small fragments of 1-3 mm3 in a cell culture dish using surgical scissors or scalpels.
5.      Digest the tissue fragments with 10mL of Tumor Tissue Digestion Solution (K601003) in a 15mL conical tube at 37°C, with variable incubation times ranging from 30 min to 1 h. Carefully monitor the digestion process, mixing the content of the tube every 5-10 min by shaking vigorously or pipetting the mixture up and down. The digestion process could be finished when most of tissue fragments are able to pass through the 1mL pipette tips.
6.      Add FBS to the tissue digestion mixture to a final concentration of 2%, and filter using a 100 μm cell strainer.
7.      Collect and centrifuge the filtered cells at 250g for 3 min at 4 °C. In case of a visible red pellet, aspirate the supernatant, and resuspend the pellet using 2mL of Red Blood Cell Lysis Solution (E238010) to lyse the erythrocytes at room temperature for 1 min and centrifuge at 250g for 3 min at 4°C.
8.      Aspirate the supernatant and resuspend the pellet in Cancer Organoid Basal Medium, centrifuge at 250g for 3 min at 4°C, repeat this step once more time.
9.      Aspirate the supernatant and resuspend the pellet in Matrigel. The Matrigel should be kept on ice to prevent it from solidifying. Perform the process as quickly as possible. The amount of Matrigel used depends on the size of the pellet. Approximately 10,000 cells should be plated in 25 μL of Matrigel.
CRITICAL: Do not overly dilute the Matrigel (Matrigel should be >70% (Matrigel vol/Total vol)), as this may inhibit the proper formation of the solid droplets.
10.   Plate the Matrigel containing organoids at the bottom of 24-well cell culture plates in droplets of ~30 μL each around the center of the well..
CRITICAL: Once the organoids are resuspended in Matrigel, proceed with plating as quickly as possible, as the Matrigel may solidify in the tube or pipette tip. Do not let the Matrigel touch the wall of wells.
11.   Place the culture plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2 for 15-25 min to let the Matrigel solidify.
12.   Prepare the required amount of endometrial cancer organoid complete medium.
13.   Once the Matrigel droplets have solidified (15-25 min), open the plate and carefully add 500 μL of organoid complete medium to each well.
CRITICAL: Do not add the medium directly on top of the Matrigel droplets, as this might disrupt the droplets.
14.   Place the culture plate in a humidified incubator at 37 °C and 5% (vol/vol) CO2.
15.   Change the medium every 3-4 d by carefully aspirating the medium from the wells and replacing it with a fresh, pre-warmed organoid complete medium.
16.   Closely monitor the organoid formation. Ideally, patient-derived endometrial cancer organoids should be passaged for the first time between 7 and 10 d after the initial plating.
Splitting and Passaging of Organoids
17.   Pipette up and down to disrupt the Matrigel and transfer the organoid suspension to a 1.5 mL conical tube.
18.   Pipette the organoid suspension up and down to mix thoroughly by pipetting against the bottom of the tube to create pressure, which will aid the removal of Matrigel.
19.   Centrifuge organoids at 250g for 3 min at room temperature.
20.   Aspirate the supernatant and split organoids using either Organoid Dissociation Solution (E238001) or by mechanical disruption. For Organoid Dissociation Solution-based cell dissociation, resuspend the pellet in 0.2 mL of Organoid Dissociation Solution, pipette up and down and incubate at 37 °C until organoids fall apart. Pipette up and down with a filter tip for ≥8 times every 2 min to aid in the disruption of the organoids. Closely monitor the digestion to keep the incubation time in the Organoid Dissociation Solution to a minimum. In case of mechanical disruption, resuspend the pellet in 1.5 mL of Cancer Organoid Basal Medium. Carefully pipette the organoid suspension up and down 30 times by pipetting against the bottom of the tube to create pressure, which will aid organoid disruption.
CRITICAL: Do not dissociate in Organoid Dissociation Solution for >7 min, as this may result in poor organoid outgrowth or even loss of the culture. As a rule of thumb, digestion is complete if a mixture of small lumps of cells (consisting of 10–50 cells) can be observed.
21.   After shearing is complete, wash once by topping up with 1 mL of Cancer Organoid Basal Medium, and centrifuge at 250g for 3 min at room temperature.
22.   Aspirate the supernatant and resuspend the organoid pellet in 70% (vol/vol) Matrigel, and plate organoids in droplets at the bottom of a culture plate as described in Steps 10. After plating is complete, transfer the plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2 for 15–25 min.
23.   Pre-warm endometrial cancer organoid complete medium at 37 °C.
24.   After the Matrigel droplets have solidified (15–25 min), carefully pipette pre-warmed medium into the wells.
Place culture plates in a humidified incubator at 37 °C and 5% (vol/vol) CO2 until the organoids are needed for further experiments.



類(lèi)器官Endometrial Cancer Organoid Kit

產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
掃碼關(guān)注 掃一掃,關(guān)注微信
版權(quán)所有©2025 普邁精醫(yī)科技(北京)有限公司    備案號(hào):京ICP備18047241號(hào)-3

化工儀器網(wǎng)    管理登陸    sitemap.xml

国产精品大屁股白浆aa| 东京热舞苍井空无码高清 | 在线看福利国语国产成人| 国产精品久久久久精囗交| 老司机一区二区在线播放| 国产精品 人妻互换| 国产一区国产二区在线视频| 欧美巨大粗爽av在线观看| 四虎成人精品在永久在线 | 日本欧美日韩亚洲综合区| 人妖免费无码av的网站| 精品久久久久久久久杏吧| 国内精品一区二区三区视频| 国产精品 高清 尿 小便 嘘嘘| 成人无码视频免费看在线| 久久久久久久久久久久久艹| 无码人妖在线看中文字幕| av亚洲av日韩av欧美| 国产青青手机在线看电影| 日本天堂亚洲欧美在线播放| 欧美18色成人黑人在线| 亚洲区一区二区三区亚洲| 亚洲国产精品一区二区制| 国产精品又爽又粗又长又硬| 午夜18禁试看120秒男女啪啪| 亚洲男人的天堂2021| 少妇高潮喷水久久久久久久久| 国内外激情免费在线视频| 国产999热这里只有精品| 日韩精品电影在线观看四区| 污色视频污色3d在线看| 99久久久亚洲精品毛片| 97成人无码免费一区二区中文| 精品久久久成人免费动漫| 亚洲最大的成人网站| 亚洲最大的中文字幕在线| 熟女精品色一区二区三区| 国产一区二区三区亚洲电影| 国产综合色婷婷精品久久| 国产欧美日本韩国一区二区| 亚洲午夜久久久久久91|